Fig 1: CGN knockout mediates the proliferation, apoptosis, migration, and invasion of OS cells induced by the miR-1270 downregulation. A) The expression of CGN. B) OS cell viability of OS cells determined by CCK-8 assay. C) The proliferation of OS cells determined by colony formation assay. D) The apoptosis of OS determined by TUNEL assay. E) The apoptosis rates determined by flow cytometry. F) The expression of Bcl-2, BAX, cleaved-Caspase-3, and cleaved-Caspase-9 determined by Western blot. G,H) The migration and invasion ability determined by transwell assay (magnification: 100x). **p<0.01 vs NC inhibitor group; ##p<0.01 vs miR-1270 inhibitor group.
Fig 2: CGN is a target of miR-1270. A) The binding site of miR-1270 on CGN. B) The luciferase activity of OS cells determined by dual reporter luciferase assay. C) The mRNA level of CGN in OS tissues and cells calculated by qRT-PCR. D) The correlation between CGN and miR-1270. E: The mRNA level of CGN in OS cells. F) The protein level of CGN in OS cells. **p<0.01 vs normal tissues, hFOB1.19, or NC inhibitor group.
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